Collingwood has ruled vice-captain Taylor Adams out of the Grand Final after he left the training track early on Tuesday with hamstring tightness.
Adams only lasted 30 minutes during the open session at Olympic Park, heading indoors and speaking to fitness staff after taking a shot at goal.
Collingwood released a statement on Tuesday afternoon saying the experienced midfielder-forward would not play.
Magpies football boss Graham Wright said the 30-year-old had “left no stone unturned” trying to make it back in time for Saturday, but the Pies could not roll the dice on him after the setback.
“Unfortunate news for Taylor Adams who experienced hamstring awareness during training today which will see him miss the Grand Final,” Wright said.
“Taylor is an ultimate professional and a true leader both on and off the field and we will wrap our arms around him and continue to support him this week.
“Taylor was incredibly eager to be out there with his teammates and left no stone unturned in his rehabilitation and recovery and we all share in his disappointment.”
But the news was more positive for Brisbane defender jack Payne who remains a chance to regain his place as he tries to overcome an ankle issue.
Payne completed Tuesday’s open training session where he was also put through extra exercises to test his ankle.
The 23-year-old will again train on Thursday before a final decision is made with the Lions grand final team being announced Thursday evening.